IRON Laboratories     PJLA

ISO/IEC 17025:2017 Accredited

Marihuana Potency Analysis by
High Performance Liquid Chromatography
Testing Accreditation #: 77802 Test Certificate #: 137410-001
Client Name, Sample Details

Holistic Healing Tim/RoseAnn
Troy, MI 48083
Sample: CBN 500mg
Type: Generic Concentrate
Method: FE04U12 HPLC-UV
Test Conditions

Prepsheet ID#: MIP220805A
Scale: XS205-MI2
Temp: 21.5 °C
Baro Pressure: 980.5 hPa
Analyst: SKM
Technician: SKM
Sample ID#: 137410

Harvest/Process Date: 08/05/2022
Serving Size (g): 9.2
Date Received: 08/05/2022
Test Date: 08/05/2022
Valid Through: 08/06/2023
Report Issued: 08/08/2022
Sample #137410
Sample #137410 Sample #137410
Test
Compounds		 Δ9-THC THCA Δ8-THC CBD CBDA CBG CBGA CBN CBC CBL THCV CBDV Total
Cannabinoids*		 Total THC Total CBD Calc Max Total
Cannabinoids*
Amount (%) 0.2 N/D N/D 6.9 N/D 0.5 N/D 6.6 0.2 N/D N/D N/D 14.4 0.2 6.9 14.4
Amount (mg/g) 2.3 N/D N/D 68.6 N/D 5.1 0.32 65.5 1.5 0.1 N/D 0.4 143.82 2.3 68.6 143.8
Amount per Serving (mg) 21.16 N/D N/D 631.12 N/D 46.92 2.944 602.6 13.8 0.92 N/D 3.68 1,323.1  
LOQ (mg/g) 0.07 0.07 0.07 0.07 0.07 0.07 0.07 0.07 0.07 0.07 0.07 0.07   %Decarb. THC CBD
±%RPD 5.14% 1.22% 2.92% 0.46% 1.15% 2.53% 0.17% 1.93% 0.52% 1.06% 0.53% 1.58% 100% 100%

Serving size = 10mL = 9.2g

LOQ = Limit of Quantitation; %RPD = Relative Percent Deviation; %RSD = Relative Standard Deviation; N/D = Not Detected

*Designates values that are not currently included in the accredited scope of Iron Laboratories.

*** Designates tests that use the method FE-45. FE-45 is performed using AOAC 966.02 and 32.004-32.009.

FE-45 has relative expanded (k=2) uncertainties of 1.098% for moisture, 1.754% for water activity for unprocessed plant materials, and 13.102% for water activity for infused products.

Vitamin E acetate analysis has a relative expanded (k=2) uncertainty of 18.614%.

Total THC and CBD is the calculated sum of Δ9-THC and Δ8-THC or CBD plus the amount of THC or CBD derived from THCA or CBDA, respectively.

These values are calculated by applying a molar correction factor of 0.877 to the THCA or the CBDA value.

Calc Max Total Cannabinoids is the sum of Total THC, Total CBD, CBN, Total CBG, CBC, THCV, CBDV, and CBL.

Total CBG is calculated as CBG plus CBGA*0.878 (the molar correction factor for CBGA to CBG conversion).

%Decarb. THC and CBD refer to the percentage of Δ9-THC or CBD relative to THCA or CBDA, respectively.

This sample has not undergone random sampling and has not been tested for compliant state, batch representative testing. These results should therefore be used for research and development or quality control purposes only. Results apply to the sample as received.

This certificate shall not be reproduced except in full, without written approval of Iron Laboratories, LLC.
KEB - LM Iron laboratories KEB - LM

Iron Laboratories, LLC is an ISO/IEC 17025:2017 Testing Laboratory laboratory accredited by (PJLA) Perry Johnson Laboratory Accreditation, Certificate No. 77802

Tested by Iron Laboratories Michigan, 1825 E. West Maple Walled Lake, MI 48390

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IRON Laboratories     PJLA

ISO/IEC 17025:2017 Accredited

QC Report
Testing Accreditation #: 77802 Test Certificate #: 137410-001
Client Name, Sample Details

Holistic Healing Tim/RoseAnn
Troy, MI 48083
Sample: CBN 500mg
Type: Generic Concentrate
Method: FE04U12 HPLC-UV
Test Conditions

Prepsheet ID#: MIP220805A
Scale: XS205-MI2
Temp: 21.5 °C
Baro Pressure: 980.5 hPa
Analyst: SKM
Technician: SKM
Sample ID#: 137410

Harvest/Process Date: 08/05/2022
Serving Size (g): 9.2
Date Received: 08/05/2022
Test Date: 08/05/2022
Valid Through: 08/06/2023
Report Issued: 08/08/2022
Sample #137410
Target Compound Name Method Blank (μg/g) LCS Spike (μg/g) LCS Amount (μg/g) Percent Recovery (%) LCS LCS Duplicate Amount (μg/g) Percent Recovery (%) LCSD Relative Percent Difference (%) QC Flag
Δ9-THC 0 4.93369 5.00168 101.38 5.26574 106.73 5.14%
THCA 0 4.71882 4.94934 104.89 5.01031 106.18 1.22%
Δ8-THC 0 5.23565 5.38002 102.76 5.53957 105.80 2.92%
CBD 0 4.94985 5.08864 102.80 5.06535 102.33 0.46%
CBDA 0 4.79149 4.98821 104.11 5.04571 105.31 1.15%
CBG 0 5.27562 5.46092 103.51 5.32433 100.92 2.53%
CBGA 0 4.92597 5.10505 103.64 5.11358 103.81 0.17%
CBN 0 5.55634 5.02161 90.38 5.11948 92.14 1.93%
CBC 0 4.9931 5.23812 104.91 5.26537 105.45 0.52%
CBCA N/A 0 0 0.00 0 0.00 0%
CBL 0 5.08023 5.30463 104.42 5.36125 105.53 1.06%
THCV 0 5.054 5.26038 104.08 5.28832 104.64 0.53%
CBDV 0 5.02113 5.21662 103.89 5.29961 105.55 1.58%

N.D. = Not Detected

LR = indicates compound recovery of matrix spike was outside the methods acceptable limits. (70-130%) Low recovery should be scrutinized for possible fail as it could indicate more compound present than is detected.

I = indicates that an amount of an interfering compound greater than the methods limit of detection was detected in the method blank sample. May indicate contamination of analytical system or consumables.

Q = indicates that the relative percent diference of two identicly prepared Matrix Spike samples for a target analyte was greater than 20%

HR = indicates compound recovery of matrix spike was outside the methods acceptable limits. (70-130%) high recoveries should be scrutinized for passing as more compound may be detected than is actually present in the sample.

This sample has not undergone random sampling and has not been tested for compliant state, batch representative testing. These results should therefore be used for research and development or quality control purposes only. Results apply to the sample as received.

This certificate shall not be reproduced except in full, without written approval of Iron Laboratories, LLC.
KEB - LM Iron laboratories KEB - LM

Iron Laboratories, LLC is an ISO/IEC 17025:2017 Testing Laboratory laboratory accredited by (PJLA) Perry Johnson Laboratory Accreditation, Certificate No. 77802

Tested by Iron Laboratories Michigan, 1825 E. West Maple Walled Lake, MI 48390

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