IRON Laboratories     PJLA

ISO/IEC 17025:2017 Accredited

Marihuana Potency Analysis by
High Performance Liquid Chromatography

Testing Accreditation #: 77802 Test Certificate #: 136697-001
Client Name, Sample Details

Holistic Healing Tim/RoseAnn
Troy, MI 48083
Sample: 1500 Tincture
Type: Tincture
Method: FE04U12 HPLC-UV

Test Conditions

Prepsheet ID#: MIP220429A
Scale: XS205-MI2
Temp: 22 °C
Baro Pressure: 970.1 hPa
Analyst: KEB
Technician: SKM

Sample ID#: 136697

Harvest/Process Date: 05/03/2022
Serving Size (g): 28.35
Date Received: 05/03/2022
Test Date: 04/29/2022
Valid Through: 04/30/2023
Report Issued: 05/03/2022

Sample #136697
Sample #136697 Sample #136697
Test
Compounds
Δ9-THC THCA Δ8-THC CBD CBDA CBG CBGA CBN CBC CBL THCV CBDV Total
Cannabinoids*
Total THC Total CBD Calc Max Total
Cannabinoids*
Amount (%) N/D N/D N/D 6.3 N/D N/D N/D N/D 0.1 N/D N/D 0.1 6.5 N/D 6.3 6.5
Amount (mg/g) N/D N/D N/D 63.3 N/D 0.3 N/D N/D 0.6 N/D N/D 0.7 64.9 N/D 63.3 64.9
Amount per Serving (mg) N/D N/D N/D 1794.555 N/D 8.505 N/D N/D 17.01 N/D N/D 19.845 1,839.9 Serving Size~ (g): 28.4
LOQ (mg/g) 0.29 0.29 0.29 0.29 0.29 0.29 0.29 0.29 0.29 0.29 0.29 0.29   %Decarb. THC CBD
±%RPD 5.05% 4.07% 3.69% 0.56% 0.34% 7.10% 2.91% 1.48% 0.50% 1.65% 1.89% 0.11% N/A 100%

Serving size = 1 oz = 28.35g.

LOQ = Limit of Quantitation; %RPD = Relative Percent Deviation; %RSD = Relative Standard Deviation; N/D = Not Detected

*Designates values that are not currently included in the accredited scope of Iron Laboratories.

*** Designates tests that use the method FE-45. FE-45 is performed using AOAC 966.02 and 32.004-32.009.

FE-45 has relative expanded (k=2) uncertainties of 1.098% for moisture, 1.754% for water activity for unprocessed plant materials, and 13.102% for water activity for infused products.

Vitamin E acetate analysis has a relative expanded (k=2) uncertainty of 18.614%.

Total THC and CBD is the calculated sum of Δ9-THC and Δ8-THC or CBD plus the amount of THC or CBD derived from THCA or CBDA, respectively.

These values are calculated by applying a molar correction factor of 0.877 to the THCA or the CBDA value.

Calc Max Total Cannabinoids is the sum of Total THC, Total CBD, CBN, Total CBG, CBC, THCV, CBDV, and CBL.

Total CBG is calculated as CBG plus CBGA*0.878 (the molar correction factor for CBGA to CBG conversion).

%Decarb. THC and CBD refer to the percentage of Δ9-THC or CBD relative to THCA or CBDA, respectively.

This sample has not undergone random sampling and has not been tested for compliant state, batch representative testing. These results should therefore be used for research and development or quality control purposes only. Results apply to the sample as received.

This certificate shall not be reproduced except in full, without written approval of Iron Laboratories, LLC.

KEB - LM Iron laboratories KEB - LM

Iron Laboratories, LLC is an ISO/IEC 17025:2017 Testing Laboratory laboratory accredited by (PJLA) Perry Johnson Laboratory Accreditation, Certificate No. 77802

Tested by Iron Laboratories Michigan, 1825 E. West Maple Walled Lake, MI 48390

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IRON Laboratories     PJLA

ISO/IEC 17025:2017 Accredited

QC Report

Testing Accreditation #: 77802 Test Certificate #: 136697-001
Client Name, Sample Details

Holistic Healing Tim/RoseAnn
Troy, MI 48083
Sample: 1500 Tincture
Type: Tincture
Method: FE04U12 HPLC-UV

Test Conditions

Prepsheet ID#: MIP220429A
Scale: XS205-MI2
Temp: 22 °C
Baro Pressure: 970.1 hPa
Analyst: KEB
Technician: SKM

Sample ID#: 136697

Harvest/Process Date: 05/03/2022
Serving Size (g): 28.35
Date Received: 05/03/2022
Test Date: 04/29/2022
Valid Through: 04/30/2023
Report Issued: 05/03/2022

Sample #136697
Target Compound Name Method Blank (μg/g) LCS Spike (μg/g) LCS Amount (μg/g) Percent Recovery (%) LCS LCS Duplicate Amount (μg/g) Percent Recovery (%) LCSD Relative Percent Difference (%) QC Flag
Δ9-THC 0 5.564257845 5.965819089 107.22 5.671758905 101.93 5.05%
THCA 0 4.905594923 5.250902568 107.04 5.041501395 102.77 4.07%
Δ8-THC 0 5.65020171 5.824372275 103.08 5.613254804 99.35 3.69%
CBD 0 5.516806491 5.591330615 101.35 5.622828118 101.92 0.56%
CBDA 0 4.953665684 5.026041514 101.46 5.009120935 101.12 0.34%
CBG 0 6.078444086 6.149979483 101.18 5.728266101 94.24 7.10%
CBGA 0 5.185222346 5.257635335 101.40 5.10702082 98.49 2.91%
CBN 0 5.330309854 5.4417865 102.09 5.36207894 100.60 1.48%
CBC 0 5.73228171 5.819432631 101.52 5.848577752 102.03 0.50%
CBCA N/A N/A N/A nan N/A nan 0%
CBL 0 5.500631052 5.965551771 108.45 5.867992834 106.68 1.65%
THCV 0 5.725777007 5.878614282 102.67 5.768430064 100.74 1.89%
CBDV 0 5.687714996 5.724518945 100.65 5.717942188 100.53 0.11%

N.D. = Not Detected

LR = indicates compound recovery of matrix spike was outside the methods acceptable limits. (70-130%) Low recovery should be scrutinized for possible fail as it could indicate more compound present than is detected.

I = indicates that an amount of an interfering compound greater than the methods limit of detection was detected in the method blank sample. May indicate contamination of analytical system or consumables.

Q = indicates that the relative percent diference of two identicly prepared Matrix Spike samples for a target analyte was greater than 20%

HR = indicates compound recovery of matrix spike was outside the methods acceptable limits. (70-130%) high recoveries should be scrutinized for passing as more compound may be detected than is actually present in the sample.

This sample has not undergone random sampling and has not been tested for compliant state, batch representative testing. These results should therefore be used for research and development or quality control purposes only. Results apply to the sample as received.

This certificate shall not be reproduced except in full, without written approval of Iron Laboratories, LLC.

KEB - LM Iron laboratories KEB - LM

Iron Laboratories, LLC is an ISO/IEC 17025:2017 Testing Laboratory laboratory accredited by (PJLA) Perry Johnson Laboratory Accreditation, Certificate No. 77802

Tested by Iron Laboratories Michigan, 1825 E. West Maple Walled Lake, MI 48390

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