IRON LaboratoriesPJLA

ISO/IEC 17025:2005 Accredited

Marihuana Potency Analysis by
High Performance Liquid Chromatography
Testing Accreditation #: 77802 Test Certificate #: 117758-001
Client Name, Sample Details

Holistic Healing Tim/RoseAnn
Troy, MI 48083
Sample: Crude CBD
Type: Tincture
Method: FE04U HPLC-UV
Test Conditions

Prepsheet ID#: MIP190703
Scale: XS205-MI2
Temp: 23.6 °C
Baro Pressure: 977.8 hPa
Analyst: KEB
Technician: KEB
Sample ID#: 117758

Harvest/Process Date: 07/05/2019
Serving Size (g): 1
Date Received: 07/05/2019
Test Date: 07/03/2019
Valid Through: 07/03/2020
Report Issued: 07/05/2019
Sample #117758
Sample #117758 Sample #117758
Test
Compounds		 THC THCA CBD CBDA CBN CBG* CBC* THCV* CBDV* Total
Cannabinoids*		 Total THC Total CBD Calc Max Total
Cannabinoids*
Amount (%) 2.6 N/D 50.8 8.2 N/D 1.0 3.0 N/D 1.0 66.6 2.6 58.0 65.6
Amount (mg/g) 25.8 N/D 508.2 81.6 N/D 9.5 30.4 N/D 10.4 665.9 25.8 579.8 655.9
Amount per Serving (mg) 25.8 N\D 508.2 81.6 N\D 9.5 30.4 N\D 10.4 665.9 Serving Size~ (g): 1.0
LOQ (mg/g) 3.3 3.3 3.3 3.3 3.3 3.3 3.3 3.3 3.3   %Decarb. THC CBD
±%RPD 4.48 2.99 1.57 0.10 1.40 1.50 2.75 1.26 3.33 100% 86

LOQ = Limit of Quantitation; %RPD = Relative Percent Deviation; %RSD = Relative Standard Deviation; N/D = Not Detected

*Designates values that are not currently included in the accredited scope of Iron Laboratories.

*** Designates tests that use the method FE-45. FE-45 is performed using AOAC 966.02 and 32.004-32.009. FE-45 has relative expanded (k=2) uncertainties of 1.098% for moisture, 1.754% for water activity for unprocessed plant materials, and 13.102% for water activity for infused products. Vitamin E acetate analysis has a relative expanded (k=2) uncertainty of 18.614%.

Total THC and CBD is the calculated sum of THC or CBD and the amount of THC or CBD derived from THCA or CBDA, respectively. These values are calculated by applying a molar correction factor of 0.877 to the THCA or the CBDA value. Calc Max Total Cannabinoids is the sum of Total THC, Total CBD, CBN, CBG, CBC, THCV, and CBDV.

%Decarb. THC and CBD refer to the percentage of THC or CBD relative to THCA or CBDA, respectively.

This certificate shall not be reproduced except in full, without written approval of Iron Laboratories, LLC.
ACR - LM Iron laboratories ACR - LM

Iron Laboratories, LLC is an ISO/IEC 17025:2005 Testing Laboratory laboratory, accredited by (PJLA) Perry Johnson Laboratory Accreditation, Certificate No. 77802

Tested by Iron Laboratories Michigan, 1825 E. West Maple Walled Lake, MI 48390

Page 1 of 1

IRON LaboratoriesPJLA

ISO/IEC 17025:2005 Accredited

QC Report
Testing Accreditation #: 77802 Test Certificate #: 117758-001
Client Name, Sample Details

Holistic Healing Tim/RoseAnn
Troy, MI 48083
Sample: Crude CBD
Type: Tincture
Method: FE04U HPLC-UV
Test Conditions

Prepsheet ID#: MIP190703
Scale: XS205-MI2
Temp: 23.6 °C
Baro Pressure: 977.8 hPa
Analyst: KEB
Technician: KEB
Sample ID#: 117758

Harvest/Process Date: 07/05/2019
Serving Size (g): 1
Date Received: 07/05/2019
Test Date: 07/03/2019
Valid Through: 07/03/2020
Report Issued: 07/05/2019
Sample #117758
Target Compound Name Method Blank (μg/g) LCS Spike (μg/g) LCS Amount (μg/g) Percent Recovery (%) LCS LCS Duplicate Amount (μg/g) Percent Recovery (%) LCSD Relative Percent Difference (%) QC Flag
Cannabidivarin (CBDV) 0 5.2221 5.22387 100.03 5.40103 103.43 3.33
Cannabidiolic Acid (CBDA) 0 5.46665 5.71418 104.53 5.72004 104.64 0.10
Cannabigerol (CBG) 0 5.60061 5.72848 102.28 5.81507 103.83 1.50
Cannabidiol (CBD) 0 5.11706 5.20725 101.76 5.28991 103.38 1.57
Δ9-Tetrahydrocannabivarin (THCV) 0 5.24494 5.51606 105.17 5.58623 106.51 1.26
Cannabinol (CBN) 0 5.20429 5.5025 105.73 5.57981 107.22 1.40
Δ9-Tetrahydrocannabinol (THC) 0 5.39666 5.627 104.27 5.88473 109.04 4.48
Cannabichromene (CBC) 0 5.25494 5.64504 107.42 5.80267 110.42 2.75
Tetrahydrocannabinolic acid (THCA) 0 5.22858 5.5674 106.48 5.73627 109.71 2.99

N.D. = Not Detected

LR = indicates compound recovery of matrix spike was outside the methods acceptable limits. (70-130%) Low recovery should be scrutinized for possible fail as it could indicate more compound present than is detected.

I = indicates that an amount of an interfering compound greater than the methods limit of detection was detected in the method blank sample. May indicate contamination of analytical system or consumables.

Q = indicates that the relative percent diference of two identicly prepared Matrix Spike samples for a target analyte was greater than 20%

HR = indicates compound recovery of matrix spike was outside the methods acceptable limits. (70-130%) high recoveries should be scrutinized for passing as more compound may be detected than is actually present in the sample.

This certificate shall not be reproduced except in full, without written approval of Iron Laboratories, LLC.
ACR - LM Iron laboratories ACR - LM

Iron Laboratories, LLC is an ISO/IEC 17025:2005 Testing Laboratory laboratory, accredited by (PJLA) Perry Johnson Laboratory Accreditation, Certificate No. 77802

Tested by Iron Laboratories Michigan, 1825 E. West Maple Walled Lake, MI 48390

Page 2 of 1